abstract：:The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Tryp...

journal_title：Experimental parasitology

authors： Saveria T,Kessler P,Jensen BC,Parsons M

更新日期：2007-05-01 00:00:00

abstract：:In this work, an ecto-phosphatase activity of Entamoeba histolytica was characterized using intact cells. This activity presented the following biochemical characteristics: (i) it hydrolyzes p-NPP with V(max) of 8.00+/-0.22 nmol p-NP x h(-1) x 10(-5) cells and K(m) of 2.68+/-0.25 mM; (ii) it is inhibited by acid phosp...

journal_title：Experimental parasitology

authors： de Sá Pinheiro AA,Amazonas JN,de Souza Barros F,De Menezes LF,Batista EJ,Silva EF,De Souza W,Meyer-Fernandes JR

更新日期：2007-04-01 00:00:00

abstract：:Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C. sinensis. The primers designed from the nucleotide sequence data der...

journal_title：Experimental parasitology

authors： Parvathi A,Sanath Kumar H,Kenchanna Prakasha B,Lu J,Xu X,Hu W,Feng Z,Karunasagar I,Karunasagar I

更新日期：2007-03-01 00:00:00

abstract：:The main goal of the present study was to characterise the course of infection and immunological responses developed by Leishmania infantum infected BALB/c mice. Parasite load was determined by Real-time TaqMan PCR while cytokine and Immunoglobulin G (IgG) production were assessed by ELISA. Leishmania DNA was detected...

journal_title：Experimental parasitology

authors： Rolão N,Cortes S,Gomes-Pereira S,Campino L

更新日期：2007-03-01 00:00:00

abstract：:Shigellosis is a serious public health problem in Korea, because large outbreaks of Shigella sonnei infections were recorded in many parts of the country during the period 1998-2000. However, the epidemiological features of shigellosis are not well known. In this study, we devised conditions suitable for the growth an...

journal_title：Experimental parasitology

authors： Jeong HJ,Jang ES,Han BI,Lee KH,Ock MS,Kong HH,Chung DI,Seol SY,Cho DT,Yu HS

更新日期：2007-02-01 00:00:00

abstract：:Environmental stress and endocrine control can affect pathogenesis of sexually transmitted diseases such as trichomoniasis. Acute Tritrichomonas foetus infection was compared in female BALB/c mice to infections in mice treated with high doses of estradiol or housed in constant bright illumination (stressed). In untrea...

journal_title：Experimental parasitology

authors： Rutkowski MR,Harmsen AG

更新日期：2007-02-01 00:00:00

abstract：:Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different classes of drugs, which were described to produce unpleasant side effects. Mentha x piperita, popularly known as peppermint, is a plant that is frequently used in the popular medicine to treat gast...

journal_title：Experimental parasitology

authors： Vidal F,Vidal JC,Gadelha AP,Lopes CS,Coelho MG,Monteiro-Leal LH

更新日期：2007-01-01 00:00:00

abstract：:An active Trypanosoma cruzi transmission cycle maintained by wild rodents in the Andean valleys of Cochabamba Bolivia is described. Wild and domestic Triatoma infestans with 60% infection with T. cruzi were found and was evidenced in 47.5% (rodents) and 26.7% (marsupial) by parasitological and/or serologycal methods. ...

journal_title：Experimental parasitology

authors： Cortez MR,Pinho AP,Cuervo P,Alfaro F,Solano M,Xavier SC,D'Andrea PS,Fernandes O,Torrico F,Noireau F,Jansen AM

更新日期：2006-12-01 00:00:00

abstract：:Trypanosoma cruzi arginine kinase is a key enzyme in cell energy management and is also involved in pH and nutritional stress response mechanisms. T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasit...

journal_title：Experimental parasitology

authors： Miranda MR,Canepa GE,Bouvier LA,Pereira CA

更新日期：2006-12-01 00:00:00

abstract：:While in medium containing glucose, schistosomes exhibit homolactic fermentation. Accumulation of lactate acid in tissue fluid causes lowering of pH and a resultant inhibition of metabolic pathways. This requires lactate transporter protein in homolactic fermentors to facilitate the translocation of lactate(-) and [H(...

journal_title：Experimental parasitology

authors： Githui EK,Damian RT,Aman RA

更新日期：2006-11-01 00:00:00

abstract：:Purine uptake has been studied in many protozoan parasites in the last few years, and several of the purine transporters have been cloned. In contrast, very little is known about the salvage of preformed pyrimidines by protozoa, and no pyrimidine transporters have been cloned, yet chemotherapy based on pyrimidine nucl...

journal_title：Experimental parasitology

authors： Gudin S,Quashie NB,Candlish D,Al-Salabi MI,Jarvis SM,Ranford-Cartwright LC,de Koning HP

更新日期：2006-10-01 00:00:00

abstract：:CBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L. amazonensis induced higher parasite burden ass...

journal_title：Experimental parasitology

authors： Veras PS,Welby-Borges M,de Santana CD,Nihei J,Cardillo F,de Freitas LA

更新日期：2006-07-01 00:00:00

abstract：AIM:The present study investigated the processes of macrogametogenesis and oocyst formation of Eimeria tenella (Xiamen strain), including the formation of wall-forming body1 (WFB1) and wall-forming body 2 (WFB2), the club-shape body and the origin of the residual body during the transformation from a macrogamete to an ...

journal_title：Experimental parasitology

authors： Kefu Z,Yingying W,Mei C,Lihong W,Shuichun H,Jun Z,Renhai L,Hong X

更新日期：2006-07-01 00:00:00

abstract：:The compound 2-hydroxy-3-(1'-propen-3-phenyl)-1,4-naphthoquinone (PHNQ6) was evaluated for activity against Toxoplasma gondii, alone or combined with sulfadiazine. Treatment with PHNQ6 combined with sulfadiazine protected at least 70 and 90% of mice infected with RH and EGS strains, respectively. Mice were treated wit...

journal_title：Experimental parasitology

authors： Ferreira RA,Oliveira AB,Ribeiro MF,Tafuri WL,Vitor RW

更新日期：2006-06-01 00:00:00

abstract：:Rosetting is a parasite adhesion phenotype associated with severe malaria in African children. Why parasites form rosettes is unknown, although enhanced invasion or immune evasion have been suggested as possible functions. Previous work showed that rosetting does not enhance parasite invasion under standard in vitro c...

journal_title：Experimental parasitology

authors： Deans AM,Rowe JA

更新日期：2006-04-01 00:00:00

abstract：:Trypanosoma rangeli is a parasite of a numerous wild and domestic animals, presenting wide geographical distribution and high immunological cross-reactivity with Trypanosoma cruzi, which may lead to misdiagnosis. T. rangeli has a complex life cycle, involving distinct morphological and functional forms in the vector. ...

journal_title：Experimental parasitology

authors： Gomes SA,Fonseca de Souza AL,Silva BA,Kiffer-Moreira T,Santos-Mallet JR,Santos AL,Meyer-Fernandes JR

更新日期：2006-04-01 00:00:00

abstract：:S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets were prepared from cultures of both axenic amast...

journal_title：Experimental parasitology

authors： Al-Mulla Hummadi YM,Al-Bashir NM,Najim RA

更新日期：2006-02-01 00:00:00

abstract：:This study was aimed to investigate the effects of both parasitism and environmental stress on the growth, reproduction, and survival of Biomphalaria alexandrina snails. Resource allocation strategies may be influenced by both biotic and abiotic factors. Using the planorbid snail B. alexandrina and Schistosoma mansoni...

journal_title：Experimental parasitology

authors： Ibrahim MM

更新日期：2006-01-01 00:00:00

abstract：:Parasites of the Leishmania genus require for the growth and viability the de novo synthesis of specific sterols as such as episterol and 5-dehydroepisterol because cholesterol, which is abundant in their mammalian hosts, does not fulfill the parasite sterol requirements. Squalene synthase catalyzes the first committe...

journal_title：Experimental parasitology

authors： Rodrigues JC,Urbina JA,de Souza W

更新日期：2005-12-01 00:00:00

abstract：:Gastro-intestinal (GI) parasites are of great agricultural importance, annually costing the livestock industry vast amounts in resources to control parasitism. One such GI parasite, Haemonchus contortus, is principally pathogenic to sheep; with the parasite's blood-feeding behaviour causing effects ranging from mild a...

journal_title：Experimental parasitology

authors： Chen Y,Donald D,Savin K,Presidente PJ,Hartman D

更新日期：2005-12-01 00:00:00

abstract：:Three-day-old specific-pathogen-free chickens were subcutaneously immunized with Eimeria tenella recombinant 5401 antigen (100 microg per chicken) with (0.25, 0.5 or 1.0mg per dose) or without ginsenosides, and boosted with the same dosage 14 days later. The chickens were challenged with 6 x 10(4) homologous sporulate...

journal_title：Experimental parasitology

authors： Du A,Hu S,Wang S

更新日期：2005-11-01 00:00:00

abstract：:Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella a...

journal_title：Experimental parasitology

authors： Talebi A,Mulcahy G

更新日期：2005-08-01 00:00:00

abstract：:One of the most fascinating aspects of the Entamoeba histolytica trophozoite ultrastructure is the lack of a typical secretory pathway, particularly of rough endoplasmic reticulum and Golgi system, in a cell with such a high secretory activity. Here, we describe the isolation of amoeba cell structures containing ER-ty...

journal_title：Experimental parasitology

authors： Salgado M,Villagómez-Castro JC,Rocha-Rodríguez R,Sabanero-López M,Ramos MA,Alagón A,López-Romero E,Sánchez-López R

更新日期：2005-08-01 00:00:00

abstract：:The infection with blood stages of Plasmodium chabaudi chabaudi (AS) was followed in BALB/c and DBA/2 mice. Both strains show a peak parasitemia by 7-9 days after infection, display splenic hypercellularity of T and B cells, thymic atrophy, nearly complete depletion of B cells in the bone marrow, and mount comparable ...

journal_title：Experimental parasitology

authors： Seixas E,Ostler D

更新日期：2005-08-01 00:00:00

abstract：:We report here the pre-mRNA 3' end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3' end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identifie...

journal_title：Experimental parasitology

authors： López-Camarillo C,Orozco E,Marchat LA

更新日期：2005-07-01 00:00:00

abstract：:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment t...

journal_title：Experimental parasitology

authors： Picazarri K,Luna-Arias JP,Carrillo E,Orozco E,Rodriguez MA

更新日期：2005-07-01 00:00:00

abstract：:It is generally accepted that a majority of individuals infected by Entamoeba histolytica do not develop symptomatic disease. However, the parasite and the host factors contributing to the development of the disease, remain undetermined. It is also unclear why certain individuals develop extra-intestinal amebiasis wit...

journal_title：Experimental parasitology

authors： Simonishvili S,Tsanava S,Sanadze K,Chlikadze R,Miskalishvili A,Lomkatsi N,Imnadze P,Petri WA Jr,Trapaidze N

更新日期：2005-07-01 00:00:00

abstract：:Activation of the hypothalamus-pituitary-adrenal axis plays a major role in the suppression of the immune system. We have investigated the effects of repetitive stress on Wistar rats infected with the Y strain of Trypanosoma cruzi and a control group that underwent stressor stimuli by exposure to ether vapor for one m...

journal_title：Experimental parasitology

authors： Santos CD,Caldeira JC,Toldo MP,Prado JC

更新日期：2005-06-01 00:00:00

abstract：:Giardia lamblia is the most commonly detected parasite in the intestinal tract of humans and other mammals causing giardiasis. Giardia presents several cytoskeletal structures with microtubules as major components such as the ventral adhesive disk, eight flagella axonemes, the median body and funis. Many drugs have al...

journal_title：Experimental parasitology

authors： Mariante RM,Vancini RG,Melo AL,Benchimol M

更新日期：2005-05-01 00:00:00

abstract：:Proteasomes are multi-subunit proteases involved in several mechanisms and thought to contribute to the regulation of cellular homeostasis. Here, we report for the first time biochemical evidence for the existence of a ubiquitin-proteasome proteolytic pathway in this parasite. Proteasomes from both cercariae and adult...

journal_title：Experimental parasitology

authors： Guerra-Sá R,Castro-Borges W,Evangelista EA,Kettelhut IC,Rodrigues V

更新日期：2005-04-01 00:00:00

abstract：:Giardia lamblia, an early diverging eukaryote that infects several species including humans and a major agent of water-borne diarrhea throughout the world, can be infected with a double-stranded RNA virus, giardiavirus (GLV). A chimeric GLV cDNA and green fluorescent protein (GFP) according to the cis-acting signals o...

journal_title：Experimental parasitology

authors： Liu Q,Zhang X,Li J,Ying J,Chen L,Zhao Y,Wei F,Wu T

更新日期：2005-03-01 00:00:00

abstract：:A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtaine...

journal_title：Experimental parasitology

authors： Kumagai T,Maruyama H,Hato M,Ohmae H,Osada Y,Kanazawa T,Ohta N

更新日期：2005-01-01 00:00:00

abstract：:Susceptible A/J and more resistant C57BL/6J mice were infected with Plasmodium chabaudi chabaudi 54X, P.c. chabaudi AS and Plasmodium chabaudi adami 408XZ. As expected, most C57BL/6J mice survived the infections with the different isolates. But in contrast to previous observations, not all A/J mice succumbed to infect...

journal_title：Experimental parasitology

authors： Hernandez-Valladares M,Naessens J,Nagda S,Musoke AJ,Rihet P,Ole-Moiyoi OK,Iraqi FA

更新日期：2004-11-01 00:00:00

abstract：:Ten pairs of Leishmania (Viannia) strains isolated from mucosal and cutaneous lesions of the same patient were analyzed genotypically in order to determine whether populations that had metastasized to mucosal sites differed from those in the cutaneous lesion. The strains were previously characterized by multi locus en...

journal_title：Experimental parasitology

authors： Cuervo P,Cupolillo E,Nehme N,Hernandez V,Saravia N,Fernandes O

更新日期：2004-09-01 00:00:00

abstract：:The N-terminal region of the cathepsin D-like aspartic protease from the human filarial parasite Onchocerca volvulus was expressed as His-tag fusion protein. Light and electron microscopic immunohistology using antibodies against the recombinant protein showed labeling of lysosomes in the hypodermis and epithelia of t...

journal_title：Experimental parasitology

authors： Jolodar A,Fischer P,Büttner DW,Miller DJ,Schmetz C,Brattig NW

更新日期：2004-07-01 00:00:00

abstract：:In the present study, several stimuli were tested to liberate Toxoplasma gondii sporozoites from oocysts. Incubation in Na(2)CO(3)-CO(2) buffer at 40 degrees C with ultrasound, followed by incubation at 40 degrees C with bile or deoxycholic acid, was the only method that resulted in 95% liberation of motile infective ...

journal_title：Experimental parasitology

authors： Freyre A,Falcón J

更新日期：2004-05-01 00:00:00

abstract：:In the present study, the gene expression of three multidrug resistance (MDR) and resistance-associated protein (MRP) transport proteins or efflux pumps was characterized and the phenotypic evidence for such pumps was demonstrated in cultured Madin-Darby canine kidney (MDCK) cells. A gradient for the fluorescent probe...

journal_title：Experimental parasitology

authors： Bonafonte MT,Romagnoli PA,McNair N,Shaw AP,Scanlon M,Leitch GJ,Mead JR

更新日期：2004-03-01 00:00:00

abstract：:Protein tyrosine phosphorylation (PY) is a well-known signalling mechanism which is also involved in host-parasite interactions. Despite its transcendence, PY has been poorly studied in parasitic helminths. The aim of this study is to examine the effect of bile salts on the PY pattern in parasitic trematodes. Two dist...

journal_title：Experimental parasitology

authors： Marcilla A,Rubia JE,Espert A,Carpena I,Esteban JG,Toledo R

更新日期：2004-01-01 00:00:00

abstract：:An efficient technique was developed for separating early and late stages of embryonic development in eggs of Heterodera glycines. This technique takes advantage of density changes that occur during embryogenesis in the developing embryo and egg to partition the egg within a sucrose step gradient. Sorted samples of eg...

journal_title：Experimental parasitology

authors： Burgwyn B,Cook RL,Bolla RI

更新日期：2003-11-01 00:00:00

abstract：:Eosinophilia in blood and tissues has been strongly associated with helminth infections for over a century. In vivo depletion of IL-5, a cytokine crucially involved in eosinophilopoiesis with an antibody or through genetic manipulation, reproducibly abrogates helminth-induced eosinophilia, but renders mice permissive ...

journal_title：Experimental parasitology

authors： Ramalingam T,Ganley-Leal L,Porte P,Rajan TV

更新日期：2003-10-01 00:00:00